These serial sections can then be reconstructed virtually in 3D. ![]() Further, using high-throughput slide scanners allows the rapid collection of high-resolution data for serial sections. Recent technological advances in microscopy now enable high-throughput imaging of thousands of cells in a short time. However, traditional stereological techniques require elaborate production and sampling of representative tissue samples, which is both time consuming and labor intensive. To date, several stereological tissue analysis methods have been developed with the aim of accurately estimating cell counts in a given tissue. Even then, manual cell counts are subjective. Manual cell counting is challenging in that it typically requires a specialist such as a biologist or a pathologist to identify and characterize different cell types. In many areas of biomedical research including clinical pathology, cell counts obtained from images are crucial data for diagnosing patients or for addressing hypotheses about developmental or pathological processes. This capacity opens the door to high-throughput 3D and 4D quantitative analyses of developmental patterns. Based on an estimated average cell size and stain color, ICS rapidly indicates the approximate location and amount of cells in histological images of labeled embryonic tissue and provides estimates of cell counts in regions with saturated fluorescence and blurred cell boundaries. We have developed the Incremental Cell Search (ICS), a novel software tool that expedites the analysis of relationships between morphological outgrowth and cell proliferation in embryonic tissues. Computerized cell localization and quantification is critical for high-throughput morphometric analysis of developing embryonic tissues. ![]() The length and tedium of traditional sampling is a major impediment to analyzing the large datasets required to accurately model cellular processes. This cellular process has traditionally been studied via sequential histological sampling of these tissues. Cell proliferation is critical to the outgrowth of biological structures including the face and limbs.
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